nonlinear regression analysis with exponential growth equation fitting graph pad prism 9.2.0  (GraphPad Software Inc)

Journal: bioRxiv

Article Title: The proteomic and transcriptomic landscapes altered by Rgg2/3 activity in Streptococcus pyogenes

doi: 10.1101/2022.05.06.490990

Figure Lengend Snippet: qRT-PCR results verifying RNA-seq and examining if proteomics targets have altered transcript levels. Wild-type NZ131, Δ rgg2 , and Δ rgg3 strains were grown in biological triplicate with or without 100 nM SHP peptide (indicated by + or – sign below each graph) and RNA was harvested late exponential phase. RNA was processed for qRT-PCR and transcript levels were determine relative to the gyrA reference gene. Significance of transcript level changes were determined using a One-way ANOVA with Multiple Comparisons Post-test. *, P < 0.05; **, P < 0.005; ****, P < 0.0005; ns, non-significant. For further experimental details, see Materials and Methods . A) Relative transcript levels of spy49_0450 (aroE.2) . B) Relative transcript levels of stcA ( spy49_0414c ). C) Relative transcript levels of slo ( spy49_0146 ). D) Relative transcript levels of sfbX49 ( spy49_1683c ). E) Relative transcript levels of upp ( spy49_0322 ). F) Relative transcript levels of spy49_0877 .

Article Snippet: Data was plotted and doubling times were calculated by taking OD 600 s ∼0.015 – 0.9 and performing a Nonlinear regression analysis with Exponential growth equation fitting with Graph Pad Prism 9.2.0 (GraphPad Software).

Techniques: Quantitative RT-PCR, RNA Sequencing